| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Lauren (8)
|
MRWEST |
33.25 |
34.58 |
1.33 |
| 2 |
Zhang, Alina C (8)
|
MRWEST |
35.76 |
35.41 |
-0.35 |
| 3 |
Weinstein, Claire J (7)
|
MRWEST |
35.48 |
35.50 |
0.02 |
| 4 |
Liu, Ava L (8)
|
MRNYSA |
35.65 |
37.23 |
1.58 |
| 5 |
Riviello, Cara M (7)
|
MRWEST |
37.24 |
37.74 |
0.50 |
| 6 |
Bhandari, Adele (7)
|
MRWEST |
38.97 |
38.19 |
-0.78 |
| 7 |
Concepcion, Alex P (8)
|
MRNYSA |
38.32 |
39.02 |
0.70 |
| 8 |
Fenton, Sofia M (8)
|
MRNYSA |
38.57 |
39.29 |
0.72 |
| 9 |
Nordstrom, Isabella N (8)
|
MRNYSA |
|
42.87 |
- |
| 10 |
Robinson, Tabitha (8)
|
NJMALT |
45.89 |
45.07 |
-0.82 |
| 11 |
Chung, Zoe E (7)
|
MRWEST |
45.68 |
46.42 |
0.74 |
| 12 |
Nordstrom, Savannah S (6)
|
MRNYSA |
|
47.16 |
- |
| 13 |
O'Mara, Erin (8)
|
MRSPAR |
49.32 |
49.51 |
0.19 |
|
Alexander, Leaila J (7)
|
MRSPAR |
55.32 |
NS |
- |